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Cultured Pearl Surface Quality Profiling by the Shell Matrix Protein Gene Expression in the Biomineralised Pearl Sac Tissue of Pinctada margaritifera ArchiMer
Blay, Carole; Planes, Serge; Ky, Chin-long.
Nucleated pearls are produced by molluscs of the Pinctada genus through the biomineralisation activity of the pearl sac tissue within the recipient oyster. The pearl sac originates from graft tissue taken from the donor oyster mantle and its functioning is crucial in determining key factors that impact pearl quality surface characteristics. The specific role of related gene regulation during gem biogenesis was unknown, so we analysed the expression profiles of eight genes encoding nacreous (PIF, MSI60, PERL1) or prismatic (SHEM5, PRISM, ASP, SHEM9) shell matrix proteins or both (CALC1) in the pearl sac (N = 211) of Pinctada margaritifera during pearl biogenesis. The pearls and pearl sacs analysed were from a uniform experimental graft with sequential...
Tipo: Text Palavras-chave: Biomineralisation; Relative gene expression; Pearl sac; Biomarkers; Pearl quality; Pinctada margaritifera.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00435/54622/56022.pdf
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Differential survivorship of congeneric ornamental fishes under forecasted climate changes are related to anaerobic potential Genet. Mol. Biol.
Gonçalves,Luciana Mara Fé; Silva,Maria de Nazaré Paula da; Val,Adalberto Luis; Almeida-Val,Vera Maria Fonseca de.
Abstract Two Amazonian closely related tetras – cardinal Paracheirodon axelrodi and green neon P. simulans – were artificially acclimatized to environmental chambers mimicking future climate change scenarios (mild, moderate and extreme), using a microcosm facility. P. simulans survived (100%) to all scenarios after 30 days exposure, while P. axelrodi presented decreasing survival percentages according to environmental severity. These differences may be the reflection of distinct natural acclimatization to microhabitats between the species, which differ in thermal conditions. Survival responses might be related to differences in relative gene expression of lactate dehydrogenase (Ldh), suggesting that P. axelrodi anaerobic potential is lower or non-existent...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ornamental fish; Enzyme activity; Relative gene expression; IPCC scenarios; Lactate Dehydrogenase (Ldh).
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100107
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Effects of local Polynesian plants and algae on growth and expression of two immune-related genes in orbicular batfish (Platax orbicularis) ArchiMer
Reverter, Miriam; Saulnier, Denis; David, Rarahu; Bardon-albaret, Agnes; Belliard, Corinne; Tapissier-bontemps, Nathalie; Lecchini, David; Sasal, Pierre.
The emerging orbicular batfish (Platax orbicularis) aquaculture is the most important fish aquaculture industry in French Polynesia. However, bacterial infections are causing severe mortality episodes. Therefore, there is an urgent need to find an effective management solution. Besides the supplying difficulty and high costs of veterinary drugs in French Polynesia, batfish aquaculture takes place close to the coral reef, where use of synthetic persistent drugs should be restricted. Medicinal plants and bioactive algae are emerging as a cheaper and more sustainable alternative to chemical drugs. We have studied the effect of local Polynesian plants and the local opportunistic algae Asparagopsis taxiformis on batfish when orally administered. Weight gain and...
Tipo: Text Palavras-chave: Orbicular batfish; Asparagopsis taxiformis; Immunostimulant; Lysozyme G; Transforming growth factor; Relative gene expression.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00350/46155/45853.pdf
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Expression of candidate genes related to metabolism, immunity and cellular stress during massive mortality in the American oyster Crassostrea virginica larvae in relation to biochemical and physiological parameters ArchiMer
Genard, Bertrand; Moraga, Dario; Pernet, Fabrice; David, Elise; Boudry, Pierre; Tremblay, Rejean.
Quantification of mRNA of genes related to metabolism, immunity and cellular stress was examined in relation to a massive mortality event during the culture of American oyster larvae, Crassostrea virginica which was probably, in regard to previous microbiological analysis, induced by vibrio infection. To document molecular changes associated with the mortality event, mRNA levels were compared to biochemical and physiological data, previously described in a companion paper. Among the 18 genes studied, comparatively to the antibiotic control, 10 showed a lower relative gene expression when the massive mortality occurred. Six of them are presumed to be related to metabolism, corroborating the metabolic depression associated with the mortality event suggested...
Tipo: Text Palavras-chave: Massive mortality; Relative gene expression; Metabolism; Immunity; Cellular stress; Larvae.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00075/18585/16138.pdf
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